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sheep anti csf1r  (R&D Systems)


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    Structured Review

    R&D Systems sheep anti csf1r
    Sheep Anti Csf1r, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 15 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+anti+m+csf/Mouse+M-CSF+R%2FCD115+Antibody/pm41891183-513-45-48
    Average 94 stars, based on 15 article reviews
    sheep anti csf1r - by Bioz Stars, 2026-09
    94/100 stars

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    Incubation:

    Article Title: Epothilone B impairs functional recovery after spinal cord injury by increasing secretion of macrophage colony-stimulating factor
    Article Snippet: .. After washing two times gently, the following primary anti-rat antibodies were incubated with the sections in the blocking buffer at 4 °C overnight: APC-conjugated anti-CD11b (1 : 100; Miltenyi, Hamburg Germany), chicken anti-GFAP (1:1000; Abcam), rabbit anti-NeuN (1 : 200; Abcam), rabbit anti-CD45 (1 : 100; Abcam), mouse anti-CD31 (1 : 100; Novus, Littleton, CO, USA), chicken anti-vimentin (1 : 5000; Novus), mouse anti-CD8 (1:100; Abcam), rabbit anti-Iba-1 (1:100; Abcam), rabbit anti-myeloperoxidase (MPO) (1:100; Abcam), mouse anti-CD68 (1 μ g/ml; Abcam), mouse anti-M-CSF (1:100; R&D), rabbit anti-M-CSF (1 : 100; Abcam) and mouse anti-O4 (1 : 200; R&D). ..

    Article Title: Epothilone B impairs functional recovery after spinal cord injury by increasing secretion of macrophage colony-stimulating factor.
    Article Snippet: .. After washing two times gently, the following primary anti-rat antibodies were incubated with the sections in the blocking buffer at 4 °C overnight: APC-conjugated anti-CD11b (1 : 100; Miltenyi, Hamburg Germany), chicken anti-GFAP (1:1000; Abcam), rabbit anti-NeuN (1 : 200; Abcam), rabbit anti-CD45 (1 : 100; Abcam), mouse anti-CD31 (1 : 100; Novus, Littleton, CO, USA), chicken anti-vimentin (1 : 5000; Novus), mouse anti-CD8 (1:100; Abcam), rabbit anti-Iba-1 (1:100; Abcam), rabbit antimyeloperoxidase (MPO) (1:100; Abcam), mouse anti-CD68 (1 μg/ml; Abcam), mouse anti-M-CSF (1:100; R&D), rabbit anti-M-CSF (1 : 100; Abcam) and mouse anti-O4 (1 : 200; R&D). ..

    Blocking Assay:

    Article Title: Epothilone B impairs functional recovery after spinal cord injury by increasing secretion of macrophage colony-stimulating factor
    Article Snippet: .. After washing two times gently, the following primary anti-rat antibodies were incubated with the sections in the blocking buffer at 4 °C overnight: APC-conjugated anti-CD11b (1 : 100; Miltenyi, Hamburg Germany), chicken anti-GFAP (1:1000; Abcam), rabbit anti-NeuN (1 : 200; Abcam), rabbit anti-CD45 (1 : 100; Abcam), mouse anti-CD31 (1 : 100; Novus, Littleton, CO, USA), chicken anti-vimentin (1 : 5000; Novus), mouse anti-CD8 (1:100; Abcam), rabbit anti-Iba-1 (1:100; Abcam), rabbit anti-myeloperoxidase (MPO) (1:100; Abcam), mouse anti-CD68 (1 μ g/ml; Abcam), mouse anti-M-CSF (1:100; R&D), rabbit anti-M-CSF (1 : 100; Abcam) and mouse anti-O4 (1 : 200; R&D). ..

    Article Title: Epothilone B impairs functional recovery after spinal cord injury by increasing secretion of macrophage colony-stimulating factor.
    Article Snippet: .. After washing two times gently, the following primary anti-rat antibodies were incubated with the sections in the blocking buffer at 4 °C overnight: APC-conjugated anti-CD11b (1 : 100; Miltenyi, Hamburg Germany), chicken anti-GFAP (1:1000; Abcam), rabbit anti-NeuN (1 : 200; Abcam), rabbit anti-CD45 (1 : 100; Abcam), mouse anti-CD31 (1 : 100; Novus, Littleton, CO, USA), chicken anti-vimentin (1 : 5000; Novus), mouse anti-CD8 (1:100; Abcam), rabbit anti-Iba-1 (1:100; Abcam), rabbit antimyeloperoxidase (MPO) (1:100; Abcam), mouse anti-CD68 (1 μg/ml; Abcam), mouse anti-M-CSF (1:100; R&D), rabbit anti-M-CSF (1 : 100; Abcam) and mouse anti-O4 (1 : 200; R&D). ..



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    Effects of miR-34a KO on expression levels of homeostatic and DAM genes. Expression levels (Heatmap) of marker genes for three different microglial stages (homeostatic, stage 1 DAM, and stage 2 DAM) are shown as fold changes miR-34a -/- to miR-34a +/+ Tg-SwDI mice, based on bulk RNAseq of the hippocampus (A) . (n=5/group, *Q < 0.05, ##Q < 0.001) Sequence alignment of the mature miR-34a with <t>CSF1R</t> 3’ untranslated region (3’UTR). The seed sequences and target mRNA are highlighted in red (B) . Effects of miR-34a KO on levels of CSF1R in lysates of the cerebral cortex were analyzed by western blotting using CSF1R antibody (C) and bar graph represents the results of densitometric analysis of CSF1R after normalizing with GAPDH levels. Data was analyzed by independent T-test. (n=4/group, ##P < 0.001).
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    Image Search Results


    Effects of miR-34a KO on expression levels of homeostatic and DAM genes. Expression levels (Heatmap) of marker genes for three different microglial stages (homeostatic, stage 1 DAM, and stage 2 DAM) are shown as fold changes miR-34a -/- to miR-34a +/+ Tg-SwDI mice, based on bulk RNAseq of the hippocampus (A) . (n=5/group, *Q < 0.05, ##Q < 0.001) Sequence alignment of the mature miR-34a with CSF1R 3’ untranslated region (3’UTR). The seed sequences and target mRNA are highlighted in red (B) . Effects of miR-34a KO on levels of CSF1R in lysates of the cerebral cortex were analyzed by western blotting using CSF1R antibody (C) and bar graph represents the results of densitometric analysis of CSF1R after normalizing with GAPDH levels. Data was analyzed by independent T-test. (n=4/group, ##P < 0.001).

    Journal: Frontiers in Immunology

    Article Title: MiR-34a deficiency enhances nucleic acid sensing and type I IFN signaling in a mouse model of Alzheimer’s disease

    doi: 10.3389/fimmu.2026.1694824

    Figure Lengend Snippet: Effects of miR-34a KO on expression levels of homeostatic and DAM genes. Expression levels (Heatmap) of marker genes for three different microglial stages (homeostatic, stage 1 DAM, and stage 2 DAM) are shown as fold changes miR-34a -/- to miR-34a +/+ Tg-SwDI mice, based on bulk RNAseq of the hippocampus (A) . (n=5/group, *Q < 0.05, ##Q < 0.001) Sequence alignment of the mature miR-34a with CSF1R 3’ untranslated region (3’UTR). The seed sequences and target mRNA are highlighted in red (B) . Effects of miR-34a KO on levels of CSF1R in lysates of the cerebral cortex were analyzed by western blotting using CSF1R antibody (C) and bar graph represents the results of densitometric analysis of CSF1R after normalizing with GAPDH levels. Data was analyzed by independent T-test. (n=4/group, ##P < 0.001).

    Article Snippet: Membranes were incubated overnight at 4 °C with primary antibodies against IFITM3 (1:1000; Abcam Cat# ab15592, RRID: AB_2122095), CSF1R (1:1000; R and D Systems Cat# AF3818, RRID: AB_884158), and GAPDH (1:5000; Millipore Cat# MAB374, RRID: AB_2107445).

    Techniques: Expressing, Marker, RNA sequencing, Sequencing, Western Blot